Coding

Part:BBa_K1781003:Design

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)


T25 - one of two fragments of the T25/T18 BACTH system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 19
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed the multiple cloning site within the T25 so that it did not interfere with the prefix and suffix


Source

The sequence was retrieved from the Euromedex BACTH System Kit

References